mTOR, the mechanistic target of rapamycin, sits at the center of a signaling network that coordinates cell growth based on environmental cues and plays an integral role in both cellular and organismal physiology.
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The Role of Revvity’s Cell Line in Supporting Wellcome Sanger Institute's genome variant exploration
A blog article demonstrating how Revvity and Wellcome Sanger Institute partnered to generate a HAP1 LIG4(-) Cas9(+) cell line in support of the Atlas of Variant Effects (AVE) alliance.
Read about Celonic's decision to partner with Revvity to leverage the CHOSOURCE™ expression platform for all their antibody and complex molecular development and manufacturing.
Colorectal cancer (CRC) is the abnormal cell growth starting in the lining of the large intestine or rectum
The Toll-like receptors (TLRs) are a family of evolutionarily ancient Patten Recognition Receptors (PRRs) that rapidly detect microbial infection and stimulate the production of pro-inflammatory and antiviral cytokines and chemokines, as well as initiating significant metabolic shifts within the cell. Acting as cellular sentinels for infectious threats, signalling downstream of TLRs sets the stage for successful development of adaptive immunity 1.
The adherens junction (AJ), with the core cadherin-catenin complex, is an integral system of cell-cell adhesion. The AJ works to anchor the actin cytoskeleton intracellularly to the plasma membrane while simultaneously linking individual cells through extracellular interactions.
When making a knockout cell line using CRISPR, western blotting is a useful tool to help in the verification of the cell line. However, correct interpretation of results is crucial. Here we summarize the possible fates of a protein after knockout and explore how best to validate a knockout cell line
A study where we have employed orthogonal target validation strategies with 3 orthogonal LOF technologies (CRISPRko, CRISPRi, and siRNA) with genes involved in maintenance of the mesenchymal-to-epithelial transition (MET).
Learn about considerations around the development of the Dharmacon™ Edit-R™ CRISPRko algorithm for functional and specific guide RNA
Reliable and consistent quality reagents are critical to experimental success and reproducibility. Learn about how Dharmacon RNA and DNA oligonucleotide synthesis delivers that level of quality, and explore troubleshooting tips for investigating unexpected results in your experiments.